2023年
12/19
開催
開催日時
2023/12/19
開催場所
Hybrid (CryoEM-Lab KEK and Zoom)
講演者
Yuichiro H. Takagi, Ph.D.
言語
English
お問い合わせ
cmasuda@post.kek.jp
概要
RNA-dependent RNA polymerases (RdRP) play critical roles in RNA-mediated gene silencing. In Arabidopsis thaliana, RNA-Dependent RNA Polymerase 2 (RDR2) generates double-stranded RNAs (dsRNAs) from a single stranded RNA template generated by DNA-dependent nuclear RNA Polymerase IV (Pol IV). Resulting dsRNAs are then processed into short-interfering RNAs that guide RNA-directed DNA methylation and transcriptional gene silencing. We determined the structure of RDR2 at 3.1 Å resolution by single-particle cryo-electron microscopy. The structure suggests its implications for double-stranded RNA synthesis in gene silencing. The seminar will also touch on several structure examples determined by Takagi lab using cryo-EM including Mediator CDK8 module from the yeast Saccharomyces cerevisiae, human asparagine synthetase, and multicopper oxidase from Marinithermus hydrothermalis.